This is a feasibility study to show that the nonreacting three-dimensional flow in the cylinder of a four-valve internal combustion engine at 160° after top dead center (atdc) at 1,500 rpm can be accurately measured by holographic particle image velocimetry. The results evidence the quality of holographic PIV measurements in engine flows and the capability of the holographic method to instantaneously capture the complete three-dimensional flow field in a large area of the highly intricate cylinder flow. The resolved measurement volume has a diameter of about 60 mm and a height of 80 mm with a vector spacing of 0.75 mm per vector. To validate the measurements, the flow structure as well as the turbulent kinetic energy of the flow field is compared with planar two-component/two-dimensional (2C/2D-PIV) measurements performed in the same engine (Dannemann et al., in Exp Fluids 2010). Furthermore, the spatial propagation of the flow field as well as the vortical structures is visualized by 3D streamlines and λ 2-contours. The current results confirm the existence of several large-scale flow structures, such as a counter-rotating ring-vortex pair below the inlet valve and the tumble vortex. The latter possesses a U-shaped propagation of the vortex core. The analysis of the two-point correlation shows the integral length scale to be in the range 2.5-6.1 mm, which is in agreement with literature data.