Isolated pseudo-RNA-recognition motifs of SR proteins can regulate splicing using a noncanonical mode of RNA recognition
Abstract
Serine/arginine (SR) proteins are key regulators of eukaryotic gene expression and have been associated with multiple human diseases including cancers. Several members of this protein family contain a noncanonical RNA recognition motif (RRM), the pseudo-RRM, for which the mode of RNA recognition is unknown. Here, we solved the structure of SRSF1 pseudo-RRM bound to RNA. It reveals the RNA motif recognized and a very unusual mode of interaction, which is conserved for all the SR proteins containing pseudo-RRMs. Finally, we show that the pseudo-RRM in isolation often is sufficient to regulate splicing, and we reveal its mechanism of action.
- Publication:
-
Proceedings of the National Academy of Science
- Pub Date:
- July 2013
- DOI:
- 10.1073/pnas.1303445110
- Bibcode:
- 2013PNAS..110E2802C