Candidate phylum TM6 genome recovered from a hospital sink biofilm provides genomic insights into this uncultivated phylum
Abstract
This research highlights the discovery and genome reconstruction of a member of the globally distributed yet uncultivated candidate phylum TM6 (designated TM6SC1). In addition to the 16S rRNA gene, no other genomic information is available for this cosmopolitan phylum. This report also introduces a mini-metagenomic approach based on the use of high-throughput single-cell genomics techniques and assembly tools that address a widely recognized issue: how to effectively capture and sequence the currently uncultivated bacterial species that make up the "dark matter of life." Amplification and sequencing random pools of 100 events enabled an estimated 90% recovery of the TM6SC1 genome.
- Publication:
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Proceedings of the National Academy of Science
- Pub Date:
- June 2013
- DOI:
- 10.1073/pnas.1219809110
- Bibcode:
- 2013PNAS..110E2390M