Interaction between human Ctf4 and the Cdc45/Mcm2-7/GINS (CMG) replicative helicase
Abstract
We describe interaction between the cell division cycle 45 (Cdc45)/minichromosome maintenance 2-7 (Mcm2-7)/Go, Ichi, Nii, and San (GINS) (the CMG complex) and chromosome transmission fidelity 4 (Ctf4). The CMG complex catalyzes parental duplex DNA separation during eukaryotic replication. Ctf4 was reported previously to bind yeast DNA polymerase α (Pol α) and be essential for human DNA replication. Here, we show that human Ctf4 (hCtf4) interacts with human CMG (hCMG) complex to form the dimeric hCtf4-monomeric hCMG complex, which, like hCMG, contains DNA helicase activity. Complex formation involves interactions of hCtf4 with multiple hCMG components. We propose that Ctf4 links the replicative Pol α-primase complex to the replicative helicase, coordinating DNA unwinding and polymerase activities.
- Publication:
-
Proceedings of the National Academy of Science
- Pub Date:
- December 2013
- DOI:
- 10.1073/pnas.1320202110
- Bibcode:
- 2013PNAS..11019760K