B-cell malignancies in microRNA Eμ-miR-17∼92 transgenic mice
Abstract
MicroRNAs (miRs) are 21- to 22-nucleotide-long noncoding RNA molecules, which regulate the expression of several genes. miR dysregulation is associated with several cancers. The MiR-17∼92 cluster is frequently upregulated in malignancies such as diffuse large B-cell lymphoma and lung cancer. To study the role of miR-17∼92 in B-cell malignancies, we developed a transgenic mouse model overexpressing this cluster in B cells. The transgenic mice developed B-cell lymphomas, and the transcriptome analysis suggested the involvement of PI3K signaling in B lymphocytes, B-cell receptor signaling, GADD45 signaling, and IL-4 signaling pathways in these disorders. Our results provide direct experimental evidence confirming that the miR-17∼92 cluster, which is amplified in human B-cell lymphoma, also is oncogenic when overexpressed in mice.
- Publication:
-
Proceedings of the National Academy of Science
- Pub Date:
- November 2013
- DOI:
- 10.1073/pnas.1315365110
- Bibcode:
- 2013PNAS..11018208S