Role of Rev-erbα domains for transactivation of the connexin43 promoter with Sp1
Abstract
Rev-erbα, a component of the circadian clock, has also been known as a nuclear receptor that lacks activation function domain 2, functioning as a ligand-dependent transcriptional repressor. However, we recently reported that Rev-erbα activates connexin43 transcription by forming a complex with Sp1. Here we show that heme, a REV-ERB ligand, is dispensable for this novel mechanism and that Rev-erbβ, having homologies with Rev-erbα, does not activate connexin43, but competes with the Rev-erbα/Sp1. The A/B region of Rev-erbα, which is not conserved in Rev-erbβ, is a crucial activating domain, while the ligand binding domain, conserved in Rev-erbβ, functions as a competitor.
- Publication:
-
FEBS Letters
- Pub Date:
- January 2013
- DOI:
- 10.1016/j.febslet.2012.11.021
- Bibcode:
- 2013FEBSL.587...98N
- Keywords:
-
- Cx43;
- connexin43;
- AB;
- A/B region;
- DBD;
- DNA binding domain;
- H;
- hinge region;
- LBD;
- ligand binding domain;
- ER;
- Estrogen Receptor;
- Connexin43;
- Nuclear receptor;
- Rev-erbα;
- Rev-erbβ;
- Sp1