Nanoscopy in a Living Mouse Brain
Abstract
We demonstrated superresolution optical microscopy in a living higher animal. Stimulated emission depletion (STED) fluorescence nanoscopy reveals neurons in the cerebral cortex of a mouse with <70-nanometer resolution. Dendritic spines and their subtle changes can be observed at their relevant scales over extended periods of time.
- Publication:
-
Science
- Pub Date:
- February 2012
- DOI:
- 10.1126/science.1215369
- Bibcode:
- 2012Sci...335..551B
- Keywords:
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- TECHNIQUES