Hyperactive self-inactivating piggyBac for transposase-enhanced pronuclear microinjection transgenesis
Abstract
We have developed a unique method for mouse transgenesis. The transposase-enhanced pronuclear microinjection (PNI) technique described herein uses the hyperactive piggyBac transposase to insert a large transgene into the mouse genome. This procedure increased transgene integration efficiency by fivefold compared with conventional PNI or intracytoplasmic sperm injection-mediated transgenesis. Our data indicate that the transposase-enhanced PNI technique additionally requires fewer embryos to be microinjected than traditional methods to obtain transgenic animals. This transposase-mediated approach is also very efficient for single-cell embryo cytoplasmic injections, offering an easy-to-implement transgenesis method to the scientific community.
- Publication:
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Proceedings of the National Academy of Science
- Pub Date:
- November 2012
- DOI:
- 10.1073/pnas.1216473109
- Bibcode:
- 2012PNAS..10919184M