Structure of the Mediator Head module bound to the carboxy-terminal domain of RNA polymerase II
Abstract
The X-ray crystal structure of the Head module, one-third of the Mediator of transcriptional regulation, has been determined as a complex with the C-terminal domain (CTD) of RNA polymerase II. The structure reveals multiple points of interaction with an extended conformation of the CTD; it suggests a basis for regulation by phosphorylation of the CTD. Biochemical studies show a requirement for Mediator-CTD interaction for transcription.
- Publication:
-
Proceedings of the National Academy of Science
- Pub Date:
- October 2012
- DOI:
- 10.1073/pnas.1215241109
- Bibcode:
- 2012PNAS..10917931R