Selective two-photon fluorescence suppression by ultrafast pulse-pair excitation: control by selective one-color stimulated emission
Abstract
Controlling two-photon molecular fluorescence leading to selective fluorophore excitation has been a long sought after goal in fluorescence microscopy. In this letter, we thoroughly explore selective fluorescence suppression through simultaneous two-photon absorption by two different fluorophores followed by selective one-photon stimulated emission for one particular fluorophore. We achieve this by precisely controlling the time delay between two identical ultrafast near infrared laser pulses.
- Publication:
-
Journal of Biomedical Optics
- Pub Date:
- October 2011
- DOI:
- Bibcode:
- 2011JBO....16j0505K
- Keywords:
-
- bio-optics;
- fluorescence;
- high-speed optical techniques;
- laser applications in medicine;
- molecular biophysics;
- optical microscopy;
- stimulated emission;
- two-photon processes;
- 87.15.mq;
- 42.62.Be;
- 87.64.mn;
- Luminescence;
- Biological and medical applications;
- Multiphoton