Nanoparticle-based protein detection by optical shift of a resonant microcavity
Abstract
We demonstrated a biosensing approach which, for the first time, combines the high sensitivity of whispering gallery modes (WGMs) with a metallic nanoparticle-based assay. We provided a computational model based on generalized Mie theory to explain the higher sensitivity of protein detection. We quantitatively analyzed the binding of a model protein (i.e., Bovine Serum Albumin) to gold nanoparticles from high-Q WGM resonance frequency shifts, and fit the results to an adsorption isotherm, which agrees with the theoretical predictions of a two-component adsorption model.
- Publication:
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Applied Physics Letters
- Pub Date:
- August 2011
- DOI:
- 10.1063/1.3599706
- arXiv:
- arXiv:1108.2337
- Bibcode:
- 2011ApPhL..99g3701S
- Keywords:
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- Physics - Optics;
- Physics - Instrumentation and Detectors
- E-Print:
- 12 pages, three figures first submitted to APL March 4 2011