Quantitative measurement of stream respiration using the resazurin-resorufin system

After three decades of active research in hydrology and stream ecology, the relationship between stream solute transport, metabolism and nutrient dynamics is still unresolved. These knowledge gaps obscure the function of stream ecosystems and how they interact with other landscape processes. To date, measuring rates of stream metabolism is accomplished with techniques that have vast uncertainties and are not spatially representative. These limitations mask the role of metabolism in nutrient processing. Clearly, more robust techniques are needed to develop mechanistic relationships that will ultimately improve our fundamental understanding of in-stream processes and how streams interact with other ecosystems. We investigated the "metabolic window of detection" of the Resazurin (Raz)-Resorufin (Rru) system (Haggerty et al., 2008, 2009). Although previous results have shown that the transformation of Raz to Rru is strongly correlated with respiration, a quantitative relationship between them is needed. We investigated this relationship using batch experiments with pure cultures (aerobic and anaerobic) and flow-through columns with incubated sediments from four different streams. The results suggest that the Raz-Rru system is a suitable approach that will enable hydrologists and stream ecologists to measure in situ and in vivo respiration at different scales, thus opening a reliable alternative to investigate how solute transport and stream metabolism control nutrient processing.