Soil Carbon Dioxide and Methane Fluxes in a Costa Rican Premontane Wet Forest

A significant amount of the global terrestrial biomass is found in tropical forests, and soil respiration is a vital part of its carbon cycling. However, data on soil trace gas flux rates in the tropics are sparse, especially from previously disturbed regions. To expand the database on carbon cycling in the tropics, this study examined soil flux rate and its variability for CO2 and CH4 in a secondary premontane wet forest south of Arenal Volcano in Costa Rica. Data were collected over a six-week period in June and July 2011 during the transition from dry to wet season. Trace gas sampling was performed at three sub-canopy sites of different elevations. The soil is of volcanic origin with a low bulk density, likely an Andisol. An average KCl pH of 4.8 indicates exchangeable aluminum is present, and a NaF pH>11 indicates the soil is dominated by short-range order minerals. Ten-inch diameter PVC rings were used as static flux chambers without soil collars. To find soil CO2 efflux rates, a battery-powered LICOR 840A CO2-H2O Gas Analyzer was used to take measurements in the field, logging CO2 concentration every ten seconds. Additionally, six, 10-mL Nylon syringes were filled with gas samples at 0, 1, 7, 14, 21, and 28 minutes after closing the chambers. These samples were analyzed the same day with a SRI 8610 Gas Chromatograph for concentrations of CO2 and CH4. The average CO2 efflux calculated was 1.7±0.8E-2 g/m2/min, and did not differ between the applied analytical methods. Soil respiration depended strongly on soil moisture, with decreasing efflux rates at higher water-filled pore space values. An annual soil respiration rate of 8.5E3 g/m2/yr was estimated by applying the observed relationship between soil moisture and CO2 efflux to annual soil moisture measurements. The relatively high respiration rates could be caused by the high soil moisture and low soil bulk density, providing optimal conditions for microbial respiration. Several diurnal sampling periods at one site showed that respiration was highest in the early evening, possibly caused by increased root respiration lagging daytime photosynthesis. Measured average CH4 flux was -7.9±6.2E-6 g/m2/min, similar to literature values; its variability was high with no temperature or soil moisture dependence discernible. However, calculated rates show that the forest was a net sink for methane, indicating that the soils were sufficiently well-drained despite high precipitation rates. Future measurements in this NSF-REU program will evaluate the role of water and root respiration in greater detail and will also incorporate sub-canopy and boundary layer gradient measurements to investigate other aspects of the carbon cycle in this environment.