Label-free quantitative cell division monitoring of endothelial cells by digital holographic microscopy
Abstract
Digital holographic microscopy (DHM) enables quantitative multifocus phase contrast imaging for nondestructive technical inspection and live cell analysis. Time-lapse investigations on human brain microvascular endothelial cells demonstrate the use of DHM for label-free dynamic quantitative monitoring of cell division of mother cells into daughter cells. Cytokinetic DHM analysis provides future applications in toxicology and cancer research.
- Publication:
-
Journal of Biomedical Optics
- Pub Date:
- May 2010
- DOI:
- 10.1117/1.3431712
- Bibcode:
- 2010JBO....15c6009K
- Keywords:
-
- biomedical optical imaging;
- brain;
- cellular biophysics;
- holography;
- optical microscopy;
- 87.16.-b;
- 87.19.L-;
- Subcellular structure and processes;
- Neuroscience