Infrared Surface Plasmon Spectroscopy of Living Cells
Abstract
We report a spectroscopic technique that combines the Fourier-Transform Infrared Spectroscopy (FTIR) with the Surface Plasmon Resonance (SPR). This FTIR-SPR technique enables sensitive infrared spectroscopy of liquid and solid objects in the attenuated total reflectance mode (ATR). The FTIR-SPR is similar to FTIR-ATR technique but has higher sensitivity due to resonance amplification of the surface electric field. FTIR-SPR is label-free method and it has unique advantages for studies of living cells. The FTIR-SPR also combines spectroscopic information inherent to FTIR with structural information provided by the Surface Plasmon Resonance (SPR). We discuss the FTIR-SPR technique for label-free studies of cell attachment to the substrate and for surface plasmon spectroscopy.
- Publication:
-
Icnaam 2010: International Conference of Numerical Analysis and Applied Mathematics 2010
- Pub Date:
- September 2010
- DOI:
- 10.1063/1.3498133
- Bibcode:
- 2010AIPC.1281.1617Y
- Keywords:
-
- plasmons;
- Fourier transform spectra;
- cellular biophysics;
- monolayers;
- 73.20.Mf;
- 33.20.Ea;
- 87.16.Gj;
- 68.18.Jk;
- Collective excitations;
- Infrared spectra;
- Cell walls;
- Phase transitions