Presence of faecal indicator bacteria in groundwaters in Kathmandu Valley, Nepal

Groundwater quality is a critical problem in Kathmandu Valley, Nepal. The population of the city increased 6 times in the last six decades and more than half of water demand depends on groundwater resource. Nevertheless, few data of microorganism presence have been reported qualitatively in the central area of the valley. We investigated distribution of faecal indicator bacteria (Total coliforms and Escherichia coli) detected in wells and analyzed the variations of the concentrations. Groundwater samples were collected from 12 deep tube wells (170-300m depths) and 36 shallow tube wells and dug wells (3-20m depths) in Aug 2008, Jan 2009, Aug 2009 and Aug 2010. River waters were also collected for analyzing effect on groundwater quality. E. coli was detected from most of all wells; the concentrations were within 1 log cfu/100mL in deep tube wells and shallow tube wells while those in dug wells ranged from 1 to 3 log cfu/100mL. E. coli was detected at extremely high level in river water, from 5 to 7 log cfu/100mL, however, no clear relation was observed between E. coli concentrations in any types of groundwaters and distance of wells from adjacent rivers. These results indicate that both types of tube wells were rather protected and dug wells were most vulnerable for faecal contamination at very local scale. Genetic analysis of bacterial communities for deep well samples showed the existence of Enterobacter, Acinetobacter as well as Methane-metabolizing groups which provide information of possible indicators other than total coliforms or E. coli for groundwater management in the valley.