MicroRNA-155 modulates the interleukin-1 signaling pathway in activated human monocyte-derived dendritic cells
Abstract
In response to inflammatory stimulation, dendritic cells (DCs) have a remarkable pattern of differentiation (maturation) that exhibits specific mechanisms to control immunity. Here, we show that in response to Lipopolysaccharides (LPS), several microRNAs (miRNAs) are regulated in human monocyte-derived dendritic cells. Among these miRNAs, miR-155 is highly up-regulated during maturation. Using LNA silencing combined to microarray technology, we have identified the Toll-like receptor/interleukin-1 (TLR/IL-1) inflammatory pathway as a general target of miR-155. We further demonstrate that miR-155 directly controls the level of TAB2, an important signal transduction molecule. Our observations suggest, therefore, that in mature human DCs, miR-155 is part of a negative feedback loop, which down-modulates inflammatory cytokine production in response to microbial stimuli.
- Publication:
-
Proceedings of the National Academy of Science
- Pub Date:
- February 2009
- DOI:
- 10.1073/pnas.0811073106
- Bibcode:
- 2009PNAS..106.2735C
- Keywords:
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- bic/miR-155;
- LPS;
- TAB2;
- TLR/IL-1 pathway;
- Biological Sciences:Immunology