Mass spectra of proteins and other biomolecules recorded using a handheld instrument
Abstract
Proteins (myoglobin and cytochrome C), peptides (bradykinin and melittin), alkaloids in complex plant materials, and mixtures of phospholipids from a bacterial extract all give characteristic electrospray mass spectra using a handheld tandem mass spectrometer, Mini 10. The mass/charge range of the 10 kg Mini 10 was extended to m/z 2000 using resonant ion ejection at low frequency allowing analysis of proteins with molecular weights up to 17,000 Da. Fragmentation of peptides and proteins was observed.
- Publication:
-
International Journal of Mass Spectrometry
- Pub Date:
- December 2008
- DOI:
- 10.1016/j.ijms.2008.04.022
- Bibcode:
- 2008IJMSp.278..166J
- Keywords:
-
- Ion trap mass spectrometer;
- Resonance ejection;
- Protein fragmentation;
- Charge state;
- Electrospray ionization;
- Fieldable instrumentation;
- On-site (in situ) analysis;
- Phospholipid;
- Alkaloid