Experiments were done in a new open cylindrical ICR cell to establish ion-ion collisions between positive and negative ions of SF6 in the gas phase for the first time. Only one electron pulse for ion generation was employed (single shot). The role of pressure was illustrated to encourage high ion densities to be a prerequisite for ion-ion reactions. No electron transfer could be deduced from the experiments. Anion-anion collisions could be indicated. The dynamics of applied potential changes on both ion polarities in a double well potential configuration was studied intensively. In depth analysis of radial excitation patterns of positive and negative ions trapped simultaneously in different stability regions reveals sharp discrimination in the extent of radial acceleration. SIMION simulations were done to probe several electric forces, which both positive and negative ions experience during radial excitation event. Axial component of radial dipolar excitation field was also analyzed. Distortion in radial ion trajectories during radial excitation event was indicated when both ion polarities were excited simultaneously.