A custom Monte Carlo (MC) platform has been established to generate opto-physiological models of mechanisms in pulse oximetry. The current research is an exploration of the process of empirically validating such a platform. With the growing availability and accuracy of tissue optical properties in literatures, MC simulation of light-tissue interaction is providing increasingly valuable information for optical bio-monitoring research. However, the extent of the validity of results from such simulations depends heavily on its agreement with empirical data. The use of images captured from a CMOS camera for the construction of intensity distributions of light transmitted through the human finger has been investigated to compare with corresponding distributions produced in the MC simulations.