cAMP controls oxygen metabolism in mammalian cells
Abstract
The impact of cAMP on ROS-balance in human and mammalian cell cultures was studied. cAMP reduced accumulation of ROS induced by serum-limitation, under conditions in which there was no significant change in the activity of scavenger systems. This effect was associated with cAMP-dependent activation of the NADH-ubiquinone oxidoreductase activity of complex I. In fibroblasts from a patient a genetic defect in the 75 kDa FeS-protein subunit of complex I resulted in inhibition of the activity of the complex and enhanced ROS production, which were reversed by cAMP. A missense genetic defect in the NDUFS4 subunit, putative substrate of PKA, suppressed, on the other hand, the activity of the complex and prevented ROS production.
- Publication:
-
FEBS Letters
- Pub Date:
- January 2006
- DOI:
- 10.1016/j.febslet.2006.06.085
- Bibcode:
- 2006FEBSL.580.4539P
- Keywords:
-
- Mitochondria;
- cAMP;
- Cellular respiration;
- ROS balance;
- Complex I