X-ray absorption spectroscopy of chloroperoxidase compound I: Insight into the reactive intermediate of P450 chemistry
Abstract
We report the structural characterization of a thiolate-ligated ferryl radical. Using x-ray absorption spectroscopy, we examined chloroperoxidase (CPO) compound I (CPO-I). Our results indicate that CPO-I is an authentic ferryl species with an Fe-O bond of 1.65 Å. Axial-ligand interactions result in a remarkably long 2.48-Å Fe-S bond. Analogous forms of cytochrome P450 and CPO have been shown to possess virtually identical coordination environments. Thus, it seems likely that our findings provide a good structural description of the elusive P450-I.
- Publication:
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Proceedings of the National Academy of Science
- Pub Date:
- November 2005
- DOI:
- 10.1073/pnas.0507069102
- Bibcode:
- 2005PNAS..10216563S
- Keywords:
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- CHEMISTRY / BIOPHYSICS