From the Cover: p600, a unique protein required for membrane morphogenesis and cell survival
Abstract
In this article, we identify and characterize p600, a unique 600-kDa retinoblastoma protein- and calmodulin-binding protein. In the nucleus, p600 and retinoblastoma protein seem to act as a chromatin scaffold. In the cytoplasm, p600 and clathrin form a meshwork structure, which could contribute to cytoskeletal organization and membrane morphogenesis. Reduced expression of p600 with interference RNA abrogates integrin-mediated ruffled membrane formation and, furthermore, prevents activation of integrin-mediated survival pathways. Consequently, knockdown of p600 sensitizes cells to apoptosis induced by cell detachment. These findings provide mechanistic insight into the regulation of membrane-proximal events in tumorigenesis.
- Publication:
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Proceedings of the National Academy of Science
- Pub Date:
- October 2005
- DOI:
- 10.1073/pnas.0507458102
- Bibcode:
- 2005PNAS..10215093N
- Keywords:
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- CELL BIOLOGY