Characterization of ABC transporter ABCB1 expressed in human neural stem/progenitor cells
Abstract
We investigated the localization and functional expression of the ABC transporter ABCB1 in human fetal neural stem/progenitor cells (hNSPCs). RT-PCR analysis revealed ABCB1 gene expression in hNSPCs. We found a single band in immunoblotted hNSPCs lysates probed with ABCB1 antibody, and detected ABCB1 at the hNSPCs cell membrane by immunocytochemistry and subcellular fractionation. ABCB1 inhibitors and substrate, and ATP-depleting agents enhanced hNSPCs' rhodamine 123 accumulation, and hNSPCs microsomes had vanadate-sensitive ATPase activity. ABCB1 and nestin expression decreased during hNSPCs differentiation, while the astroglial marker GFAP increased. ABCB1 may maintain hNSPCs in an undifferentiated state and could be a neural stem/progenitor marker.
- Publication:
-
FEBS Letters
- Pub Date:
- January 2005
- DOI:
- 10.1016/j.febslet.2005.05.019
- Bibcode:
- 2005FEBSL.579.3473I
- Keywords:
-
- ABC transporter;
- ATP-binding cassette transporter;
- MDR;
- multidrug resistance;
- P-gp;
- P-glycoprotein;
- hNSPCs;
- human neural stem/progenitor cells;
- SP;
- side-population;
- Rh123;
- rhodamine 123;
- ABC transporter;
- ABCB1;
- Neural stem cell;
- Cell surface marker