Searching for traces of life in subglacial Lake Vostok (Antarctica) in terms of forward contamination: the lessons for exploration of icy environments on Mars
Abstract
Bacterial 16S ribosomal gene analysis guarded by criteria for trace DNA analysis and Ancient DNA research clearly testifies for the very low biomass in accretion ice from giant subglacial Lake Vostok buried beneath 4-km thick East Antarctic ice sheet. It seems that the accretion ice is essentially germ-free indicating that the water body should also be hosting a highly sparse life, if any, unless the lake water lost its biological contents during accretion process. Due to this the search for life in Lake Vostok is constrained by a high chance of contamination similar to forward-contamination upon searching for life on Mars and other icy planets. Of 16 bacterial phylotypes initially recovered from the accretion ice the only one was kept with confident relevance to the lake environment while 15 others were presumed to be contaminants on the basis of indexing contaminant criteria developed for Lake Vostok and similar icy environments. The current way to avoid contamination appears to use stringent ice chemistry-based decontamination procedures and comprehensive biological controls including establishment of contemporary contaminant database as a prerequisite to identify and categorize sources of contaminants. More challenge would be to advance cleanliness and sterilization approaches and procedures in order to achieve and measure the level of cleanliness appropriate for tools exploring environments like Lake Vostok. As a guide for searching for life in (sub)glacial environments on Earth or Mars and Jovian's Europa our recommendations can be summarized as follows: (i) apply stringent ice decontamination procedures to meet chemistry and trace DNA analysis standards, (ii) document biological contents of various environments including humans in contact with ice samples (development of contaminant database), (iii) ensure in using relevant methods to cover both known and expected biodiversity and (iv) verify microbial findings through their possible metabolic profiles taking into account known physical and chemical features of an icy environment (e.g. for Lake Vostok - no light, close to freezing point temperature, low DOC and other nutrients contents, excess of oxygen and nitrogen).
- Publication:
-
35th COSPAR Scientific Assembly
- Pub Date:
- 2004
- Bibcode:
- 2004cosp...35..676B