An integrated structural, anisotropy of magnetic susceptibility (AMS) and paleomagnetic study was carried out on Plio-Pleistocene sedimentary basins in eastern Sicily. These basins belong to three main tectonic domains: the Tyrrhenian hinterland domain, the Catania foredeep domain, and the Hyblean foreland domain. We sampled 329 oriented samples from 25 sites in selected areas from the different tectonic domains. The AMS is typical for weakly deformed sediments, with a magnetic foliation sub-parallel to the bedding plane, and a well-defined magnetic lineation. The orientation of the magnetic lineation is strongly controlled by the main tectonic deformation recorded in the basins. Structural and AMS data define a transition from NW-SE extension in the Tyrrhenian hinterland domain, to E-W compression in the Catania foredeep domain, to E-W extension in the Hyblean foreland domain, respectively. Reliable paleomagnetic results have been obtained in 12 out of 25 sampled sites. Data show that no significant rotations occurred in any of the studied basins at least since the middle Pleistocene. These results allow us to define an upper limit to the large rotations about vertical axes that have been previously found in the Calabria and Sicily regions.