Yeast Life-Span Extension by Calorie Restriction Is Independent of NAD Fluctuation
Abstract
Calorie restriction (CR) slows aging in numerous species. In the yeast Saccharomyces cerevisiae, this effect requires Sir2, a conserved NAD+-dependent deacetylase. We report that CR reduces nuclear NAD+ levels in vivo. Moreover, the activity of Sir2 and its human homologue SIRT1 are not affected by physiological alterations in the NAD+:NADH ratio. These data implicate alternate mechanisms of Sir2 regulation by CR.
- Publication:
-
Science
- Pub Date:
- December 2003
- DOI:
- 10.1126/science.1088697
- Bibcode:
- 2003Sci...302.2124A
- Keywords:
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- CELL BIOL