``Plugging into Enzymes'': Nanowiring of Redox Enzymes by a Gold Nanoparticle
Abstract
The reconstitution of an apo-flavoenzyme, apo-glucose oxidase, on a 1.4-nanometer gold nanocrystal functionalized with the cofactor flavin adenine dinucleotide and integrated into a conductive film yields a bioelectrocatalytic system with exceptional electrical contact with the electrode support. The electron transfer turnover rate of the reconstituted bioelectrocatalyst is ~5000 per second, compared with the rate at which molecular oxygen, the natural cosubstrate of the enzyme, accepts electrons (~700 per second). The gold nanoparticle acts as an electron relay or ``electrical nanoplug'' for the alignment of the enzyme on the conductive support and for the electrical wiring of its redox-active center.
- Publication:
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Science
- Pub Date:
- March 2003
- DOI:
- 10.1126/science.1080664
- Bibcode:
- 2003Sci...299.1877X
- Keywords:
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- CHEMISTRY