Investigation of metal ion accumulation in Euglena gracilis by fluorescence methods
Abstract
Single cell synchrotron X-ray fluorescence (SXRF) microprobe measurements as well as X-ray absorption near edge structure experiments have been done at Beijing Synchrotron Radiation Facility on Euglena Gracilis cells. Concentrations of the metal ions Mn 2+, Nd 3+, Ce 3+ and other trace elements, such as Ca, Fe, Zn, etc. have been measured both by single cell SXRF and bulk PIXE technique. It was found that the content of Ca, Fe and Zn was lower after the uptake of rare earths or Mn by the cells, while the valence states of Mn 2+, Ce 3+ and Nd 3+ were unaltered. The results related to cytochemistry are also discussed.
- Publication:
-
Nuclear Instruments and Methods in Physics Research B
- Pub Date:
- April 2002
- DOI:
- 10.1016/S0168-583X(01)01132-6
- Bibcode:
- 2002NIMPB.189..506S