Communication of the Position of Exon-Exon Junctions to the mRNA Surveillance Machinery by the Protein RNPS1
Abstract
In mammalian cells, splice junctions play a dual role in mRNA quality control: They mediate selective nuclear export of mature mRNA and they serve as a mark for mRNA surveillance, which subjects aberrant mRNAs with premature termination codons to nonsense-mediated decay (NMD). Here, we demonstrate that the protein RNPS1, a component of the postsplicing complex that is deposited 5' to exon-exon junctions, interacts with the evolutionarily conserved human Upf complex, a central component of NMD. Significantly, RNPS1 triggers NMD when tethered to the 3' untranslated region of β-globin mRNA, demonstrating its role as a subunit of the postsplicing complex directly involved in mRNA surveillance.
- Publication:
-
Science
- Pub Date:
- September 2001
- DOI:
- 10.1126/science.1062786
- Bibcode:
- 2001Sci...293.1836L
- Keywords:
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- MOLEC BIO