Binding of tenascin-X to decorin
Abstract
Tenascin-X (TN-X) is an extracellular matrix protein whose absence results in an alteration of the mechanical properties of connective tissue. To understand the mechanisms of integration of TN-X in the extracellular matrix, overlay blot assays were performed on skin extracts. A 100 kDa molecule interacting with TN-X was identified by this method and this interaction was abolished when the extract was digested by chondroitinase. By solid-phase assays, we showed that dermatan sulfate chains of decorin bind to the heparin-binding site included within the fibronectin-type III domains 10 and 11 of TN-X. We thus postulate that the association of TN-X with collagen fibrils is mediated by decorin and contributes to the integrity of the extracellular network.
- Publication:
-
FEBS Letters
- Pub Date:
- January 2001
- DOI:
- 10.1016/S0014-5793(01)02361-4
- Bibcode:
- 2001FEBSL.495...44E
- Keywords:
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- Extracellular matrix;
- Ehlers–Danlos syndrome;
- Proteoglycan;
- Tenascin-X