VirB/D4-Dependent Protein Translocation from Agrobacterium into Plant Cells
Abstract
The Agrobacterium VirB/D4 transport system mediates the transfer of a nucleoprotein T complex into plant cells, leading to crown gall disease. In addition, several Virulence proteins must somehow be transported to fulfill a function in planta. Here, we used fusions between Cre recombinase and VirE2 or VirF to directly demonstrate protein translocation into plant cells. Transport of the proteins was monitored by a Cre-mediated in planta recombination event resulting in a selectable phenotype and depended on the VirB/D4 transport system but did not require transferred DNA.
- Publication:
-
Science
- Pub Date:
- November 2000
- DOI:
- 10.1126/science.290.5493.979
- Bibcode:
- 2000Sci...290..979V
- Keywords:
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- BOTANY