A Myosin I Isoform in the Nucleus
Abstract
A nuclear isoform of myosin I β that contains a unique 16-amino acid amino-terminal extension has been identified. An affinity-purified antibody to the 16-amino acid peptide demonstrated nuclear staining. Confocal and electron microscopy revealed that nuclear myosin I β colocalized with RNA polymerase II in an α-amanitin- and actinomycin D-sensitive manner. The antibody coimmunoprecipitated RNA polymerase II and blocked in vitro RNA synthesis. This isoform of myosin I β appears to be in a complex with RNA polymerase II and may affect transcription.
- Publication:
-
Science
- Pub Date:
- October 2000
- DOI:
- 10.1126/science.290.5490.337
- Bibcode:
- 2000Sci...290..337P
- Keywords:
-
- CELL BIOL