Structure of the Cytoplasmic β Subunit-T1 Assembly of Voltage-Dependent K+ Channels
Abstract
The structure of the cytoplasmic assembly of voltage-dependent K+ channels was solved by x-ray crystallography at 2.1 angstrom resolution. The assembly includes the cytoplasmic (T1) domain of the integral membrane α subunit together with the oxidoreductase β subunit in a fourfold symmetric T14β4 complex. An electrophysiological assay showed that this complex is oriented with four T1 domains facing the transmembrane pore and four β subunits facing the cytoplasm. The transmembrane pore communicates with the cytoplasm through lateral, negatively charged openings above the T14β4 complex. The inactivation peptides of voltage-dependent K+ channels reach their site of action by entering these openings.
- Publication:
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Science
- Pub Date:
- July 2000
- DOI:
- 10.1126/science.289.5476.123
- Bibcode:
- 2000Sci...289..123G
- Keywords:
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- BIOCHEM