Salmonella Pathogenicity Island 2-Dependent Evasion of the Phagocyte NADPH Oxidase
Abstract
A type III protein secretion system encoded by Salmonella pathogenicity island 2 (SPI2) has been found to be required for virulence and survival within macrophages. Here, SPI2 was shown to allow Salmonella typhimurium to avoid NADPH oxidase-dependent killing by macrophages. The ability of SPI2-mutant bacteria to survive in macrophages and to cause lethal infection in mice was restored by abrogation of the NADPH oxidase-dependent respiratory burst. Ultrastructural and immunofluorescence microscopy demonstrated efficient localization of the NADPH oxidase in the proximity of vacuoles containing SPI2-mutant but not wild-type bacteria, suggesting that SPI2 interferes with trafficking of oxidase-containing vesicles to the phagosome.
- Publication:
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Science
- Pub Date:
- March 2000
- DOI:
- 10.1126/science.287.5458.1655
- Bibcode:
- 2000Sci...287.1655V
- Keywords:
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- MICROBIO