The Zα domain of the editing enzyme dsRNA adenosine deaminase binds left-handed Z-RNA as well as Z-DNA
Abstract
The Zα domain of human double-stranded RNA adenosine deaminase 1 binds specifically to left-handed Z-DNA and stabilizes the Z-conformation. Here we report spectroscopic and analytical results that demonstrate that Zα can also stabilize the left-handed Z-conformation in double-stranded RNA. Zα induces a slow transition from the right-handed A-conformation to the Z-form in duplex r(CG)6, with an activation energy of 38 kcal mol-1. We conclude that Z-RNA as well as Z-DNA can be accommodated in the tailored binding site of Zα. The specific binding of Z-RNA by Zα may be involved in targeting double-stranded RNA adenosine deaminase 1 for a role in hypermutation of RNA viruses.
- Publication:
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Proceedings of the National Academy of Science
- Pub Date:
- December 2000
- DOI:
- 10.1073/pnas.240464097
- Bibcode:
- 2000PNAS...9713532B
- Keywords:
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- Biochemistry