A Small Nucleolar RNA:Ribozyme Hybrid Cleaves a Nucleolar RNA Target in vivo with near-Perfect Efficiency
Abstract
A hammerhead ribozyme has been localized to the yeast nucleolus by using the U3 small nucleolar RNA as a carrier. The hybrid small nucleolar RNA:ribozyme, designated a "snorbozyme," is metabolically stable and cleaves a target U3 RNA with nearly 100% efficiency in vivo. This is the most efficient in vivo cleavage reported for a trans-acting ribozyme. A key advantage of the model substrate featured is that a stable, trimmed cleavage product accumulates. This property allows accurate kinetic measurements of authentic cleavage in vivo. The system offers new avenues for developing effective ribozymes for research and therapeutic applications.
- Publication:
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Proceedings of the National Academy of Science
- Pub Date:
- June 1999
- DOI:
- 10.1073/pnas.96.12.6609
- Bibcode:
- 1999PNAS...96.6609S