Observation of Strange Kinetics in Protein Folding
Abstract
Highly nonexponential folding kinetics in aqueous solution have been observed during temperature jump-induced refolding of two proteins, yeast phosphoglycerate kinase and a ubiquitin mutant. The observations are most easily interpreted in terms of downhill folding, which posits a heterogeneous ensemble of structures en route to the folded state. The data are also reconciled with exponential kinetics measured under different experimental conditions and with titration experiments indicating cooperative folding.
- Publication:
-
Proceedings of the National Academy of Science
- Pub Date:
- May 1999
- DOI:
- 10.1073/pnas.96.11.6031
- Bibcode:
- 1999PNAS...96.6031S