Genotyping of human cytochrome P450 2A6 (CYP2A6), a nicotine C-oxidase
Abstract
Cytochrome P450 2A6 (CYP2A6) is a polymorphic enzyme responsible for the oxidation of certain precarcinogens and drugs and is the major nicotine C-oxidase. The role of CYP2A6 for nicotine elimination was emphasised recently by the finding that smokers carrying defective CYP2A6 alleles consumed fewer cigarettes [Pianezza et al. (1998) Nature 393, 750]. The method used for CYP2A6 genotyping has, however, been found to give erroneous results with respect to the coumarin hydroxylase phenotype, a probe reaction for the CYP2A6 enzyme. The present study describes an allele-specific PCR genotyping method that identifies the major defective CYP2A6 allele and accurately predicts the phenotype. An allele frequency of 1–3% was observed in Finnish, Spanish, and Swedish populations, much lower than described previously.
- Publication:
-
FEBS Letters
- Pub Date:
- January 1998
- DOI:
- 10.1016/S0014-5793(98)01297-6
- Bibcode:
- 1998FEBSL.438..201O
- Keywords:
-
- Cytochrome P450;
- Coumarin;
- Allele;
- Genotype;
- Phenotype;
- Methoxyflurane;
- CYP or P450;
- cytochrome P450;
- PM;
- poor metaboliser;
- PCR;
- polymerase chain reaction