Microvolume dynamic light scattering and simultaneous video microscopic observation for screening of protein crystal growth
Abstract
Dynamic light scattering (DLS), video microscopic and ultra- microscopic observation were simultaneously employed for screening of protein crystal growth in the vapor diffusion regime. The setup, consisting of a modified microscope equipped with a laser sheet (darkfield) illumination for ultra-microscopy, allows the visualization of clusters in the nanometer range as well as of macroscopic crystals parallel with the DLS measurements. The results of microscopic and ultra-microscopic observations, the DLS autocorrelation functions and the resulting relaxation time distributions for the standard protein lysozyme are presented. The special conditions for dynamic light scattering measurements in the pre and post crystallization phase with samples situated in small droplets and covered by a standard crystallization plate are discussed.
- Publication:
-
Biomedical Systems and Technologies II
- Pub Date:
- December 1997
- Bibcode:
- 1997SPIE.3199..299W