IRAK: A Kinase Associated with the Interleukin-1 Receptor
Abstract
The pleiotropic biological activities of interleukin-1 (IL-1) are mediated by its type I receptor (IL-1RI). When the ligand binds, IL-1RI initiates a signaling cascade that results in the activation of the transcription regulator nuclear factor kappa B (NF-kappaB). A protein kinase designated IRAK (IL-1 receptor-associated kinase) was purified, and its complementary DNA was molecularly cloned. When human embryonic kidney cells (cell line 293) over-expressing IL-1RI or HeLa cells were exposed to IL-1, IRAK rapidly associated with the IL-1RI complex and was phosphorylated. The primary amino acid sequence of IRAK shares similarity with that of Pelle, a protein kinase that is essential for the activation of a NF-kappaB homolog in Drosophila.
- Publication:
-
Science
- Pub Date:
- February 1996
- DOI:
- 10.1126/science.271.5252.1128
- Bibcode:
- 1996Sci...271.1128C