Z-DNA-Forming Sites within the Human β -Globin Gene Cluster
Abstract
Agarose-encapsulated, metabolically active, permeabilized nuclei from human hematopoietic cell lines were tested for Z-DNA formation in the beta-globin gene cluster. Biotinylated monoclonal antibodies against Z-DNA were diffused into the nuclei and cross-linked to DNA with a 10-ns laser exposure at 266 nm. Following digestion with restriction enzymes, fragments that had formed Z-DNA were isolated. Seventeen regions with Z-DNA sequence motifs in the 73-kb region were studied by PCR amplification, and five were found in the Z conformation.
- Publication:
-
Proceedings of the National Academy of Science
- Pub Date:
- January 1996
- DOI:
- 10.1073/pnas.93.2.780
- Bibcode:
- 1996PNAS...93..780M