Characterization and inhibition of a cholecystokinin-inactivating serine peptidase
Abstract
A cholecystokinin (CCK)-inactivating peptidase was purified and identified as a membrane-bound isoform of tripeptidyl peptidase II (EC 3.4.14.10), a cytosolic subtilisin-like peptidase of previously unknown functions. The peptidase was found in neurons responding to cholecystokinin, as well as in non-neuronal cells. Butabindide, a potent and specific inhibitor, was designed and shown to protect endogenous cholecystokinin from inactivation and to display pro-satiating effects mediated by the CCKA receptor.
- Publication:
-
Nature
- Pub Date:
- April 1996
- DOI:
- 10.1038/380403a0
- Bibcode:
- 1996Natur.380..403R