Structure and mechanism of DNA topoisomerase II
Abstract
The crystal structure of a large fragment of yeast type II DNA topoisomerase reveals a heart-shaped dimeric protein with a large central hole. It provides a molecular model of the enzyme as an ATP-modulated clamp with two sets of jaws at opposite ends, connected by multiple joints. An enzyme with bound DNA can admit a second DNA duplex through one set of jaws, transport it through the cleaved first duplex, and expel it through the other set of jaws.
- Publication:
-
Nature
- Pub Date:
- January 1996
- DOI:
- 10.1038/379225a0
- Bibcode:
- 1996Natur.379..225B