Signalling downstream of activated mammalian Notch
Abstract
NOTCH belongs to a family of transmembrane proteins that are widely conserved from flies to vertebrates and are thought to be involved in cell-fate decisions. In Drosophila, the Suppressor of hairless (Su(H)) gene1,2 and genes of the Enhancer of split (E(Spl)) complex, which encode proteins of the basic helix-loop-helix type3,4 have been implicated in the Notch signalling pathway. Mammalian homologues of E(Spl), such as the mouse Hairy enhancer of split (HES-1 )5, have been isolated. Both HES-1 and the intracellular domain of murine Notch (mNotch) are able to block MyoD-induced myogenesis5-7. Here we show that activated forms of mNotch associate with the human analogue of Su(H), KBF2/RBP-JK (refs 8, 9) and act as transcriptional activators through the KBF2-binding sites of the HES-1 promoter.
- Publication:
-
Nature
- Pub Date:
- September 1995
- DOI:
- 10.1038/377355a0
- Bibcode:
- 1995Natur.377..355J