Structural determinants of nuclear receptor assembly on DNA direct repeats
Abstract
Nuclear receptor heterodimers recognize response elements composed of two direct repeats of the consensus sequence 5'-AGGTCA-3' separated by one to five base pairs. The 1.9 Å crystal structure of the complex formed by the DNA-binding domains of the 9-cis retinoic acid receptor and thyroid hormone receptor bound to a thyroid-response element shows that the subunits interact through a DNA-supported interface involving the carboxy-terminal extension of the DNA-binding domain of the thyroid hormone receptor. The stereochemistry suggests a mechanism by which heterodimers recognize the inter-half-site spacing between direct repeats.
- Publication:
-
Nature
- Pub Date:
- May 1995
- DOI:
- 10.1038/375203a0
- Bibcode:
- 1995Natur.375..203R