Cytochrome P450 CYP3A5 in the human anterior pituitary gland
Abstract
The cytochromes P450 are a key group of enzymes involved in the metabolism of xenobiotics and several biologically active endogenous compounds. The expression of CYP3A5 has been identified by reverse transcriptase-polymerase chain reaction in human pituitary gland and shown by immunohistochemistry to be localized to growth hormone containing cells of the anterior pituitary gland. This is the first direct identification of an individual P450 subfamily in the pituitary gland and the presence of CYP3A in the pituitary gland may play a role in regulating growth hormone secretion.
- Publication:
-
FEBS Letters
- Pub Date:
- January 1995
- DOI:
- 10.1016/0014-5793(95)00367-I
- Bibcode:
- 1995FEBSL.364...79M
- Keywords:
-
- Cytochrome P450;
- Pituitary gland;
- Somatotroph;
- EPC;
- diethyl pyrocarbonate;
- P450;
- cytochrome P450;
- CYP 3A5;
- the 5th member of the cytochrome P450 family 3A;
- FITC;
- fluorescein isothiocyanate;
- RT-PCR;
- reverse transcriptase polymerase chain reaction;
- TBS;
- 0.05 M Tris-HCl-buffered saline pH 7.6