Neutrophil and B Cell Expansion in Mice That Lack the Murine IL-8 Receptor Homolog
Abstract
Interleukin-8 (IL-8) is a proinflammatory cytokine that specifically attracts and activates human neutrophils. A murine gene with a high degree of homology to the two known human IL-8 receptors was cloned and then deleted from the mouse genome by homologous recombination in embryonic stem (ES) cells. These mice, although outwardly healthy, had lymphadenopathy, resulting from an increase in B cells, and splenomegaly, resulting from an increase in metamyelocytes, band, and mature neutrophils. Thus, this receptor may participate in the expansion and development of neutrophils and B cells. This receptor was the major mediator of neutrophil migration to sites of inflammation and may provide a potential therapeutic target in inflammatory disease.
- Publication:
-
Science
- Pub Date:
- July 1994
- DOI:
- 10.1126/science.8036519
- Bibcode:
- 1994Sci...265..682C