Tumor Cell Growth Arrest Caused by Subchromosomal Transferable DNA Fragments from Chromosome 11
Abstract
A fundamental problem in the identification and isolation of tumor suppressor and other growth-inhibiting genes is the loss of power of genetic complementation at the subchromosomal level. A direct genetic strategy was developed to isolate subchromosomal transferable fragments (STFs) from any chromosome, each containing a selectable marker within the human DNA, that could be transferred to any mammalian cell. As a test of the method, several overlapping STFs from 11p15 were shown to cause in vitro growth arrest of rhabdomyosarcoma cells. This activity mapped between the β-globin and insulin genes.
- Publication:
-
Science
- Pub Date:
- April 1993
- DOI:
- 10.1126/science.8469989
- Bibcode:
- 1993Sci...260..361K