Peptide sequencing identifies MSS1, a modulator of HIV Tat-mediated transactivation, as subunit 7 of the 26 S protease
Abstract
Subunit 7 is an integral component of the human erythrocyte 26 S protease. Peptide sequence analysis reveals that 22 amino acids from the N-terminus of subunit 7 correspond exactly to the N-terminus of MSS1, a modulator of HIV gene expression. Additional internal peptides from subunit 7 obtained by CNBr cleavage also match 100% with the deduced amino acid sequence of MSS1. Based on the fact that directly sequenced peptides from subunit 7 are identical to more than 12% of the hypothetical translation product of MSS1, and the fact that the molecular weight of subunit 7 (49 kDa) corresponds to the predicted molecular weight of MSS1 (48,633 Da), we conclude that subunit 7 is MSS1.
- Publication:
-
FEBS Letters
- Pub Date:
- January 1993
- DOI:
- 10.1016/0014-5793(93)81356-5
- Bibcode:
- 1993FEBSL.323..276D
- Keywords:
-
- MSS1;
- HIV;
- Human 26 S protease: Putative ATPase;
- Tat;
- SDS;
- sodium dodecyl sulfate;
- PAGE;
- polyacrylamide gel electrophoresis;
- HIV;
- human immunodeficiency virus;
- sLLVY-MCA;
- succinyl-Leu-Leu-Val-Tyr-7-amido-4-methyl coumarin;
- CNBr;
- cyanogen bromide;
- PVDF;
- polyvinylidene difluoride.